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Raw data for all yeast genome microarrays used in this study are available here as well as on the Stanford
Microarray
Database
For each microarray, two scan
files (TIF files) were generated, one for each fluorescence emission
wavelength
corresponding to the fluorophor used in the reverse transcription labeling
reaction (i.e. Cy3-dUTP = 532 nm, Cy5-dUTP = 632 nm). Image analysis for
each array was performed on IBM PC-compatible computers using ScanAlyze, and included a
semi-manual gridding process whereby the spots were identified
in a grid file (SAG file). The extracted dat files are
available as text tab-delimited ScanAlyze output files (DAT
files).
The data for each spot on the array are displayed in each row of
the DAT file. Gene identification for each spot can be accessed in the
accompanying Genelist file. Each array can also be viewed through the
accompanying GIF image.
You can also download the expression ratios from all of
the arrays (log2 ratios), available in a
text tab-delimited cdt file. The data in this
file can be organized by a hierarchical clustering algorithm (Eisen et al., 1998) using the
program Cluster, a software package written by
Michael Eisen. The resulting data can be viewed using the Windows program Treeview, also written by Michael Eisen.
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